Combined analysis of colloidal and conformational stability: Combine the forces of classical DLS and nanoDSF protein unfolding analysis to shed light on the most important pathways of protein dysfunctionalization.Deep information content: Get insights into sample quality, abundant species distribution, effects of ligand binding and conformational changes in one experiment.Sizes down to 1 nm and up to 2 µm can be reliably detected: Peptides, proteins, nucleic acids, lipids and liposomes, artifical polymers, detergents and detergent micelles, viruses and virus-like particles, cell organelles. Great sample type versatility: All kinds of macromolecular and polymeric sample types can be measured.This also enables high-throughput analysis of 100s to 1000s of conditions for (pre-)formulation development. Short measurement time: 48 samples require only 20 minutes for isothermal DLS measurement or just one hour for thermal ramp measurement.Comparability studies (determine particle size distribution across different batches, lots, processes, perform stress testing of biologics)įor more information on these applications or the DLS analysis services by 2bind, take a look at our Application Database or contact our DLS specialists.ĭLS in general and with the Prometheus Panta in particular offers a great number of benefits:.Small molecule quality control (determine particle sizes after reconstituion, dilution, mixing, isothermally and across temperature gradients).Biologics development (characterize protein unfolding and aggregation, get self- and non-specific interactions, analyze protein size and homogeneity).Process development (determine and compare protein aggregation, characterize particle sizing and size distribution during process scale-up and optimization).Pre-formulation and formulation development (determine aggregation propensity, determine thermal stability, perform buffer and excipient screening, characterize particle sizing and solution homogeneity).Antibody engineering (determine aggregation propensity, get self- and non-specific interactions).Thus a broad image of comprehensive protein stability pathways can be obtained from a single experiment.ĭue to the robust detection method and quick analysis pace, Prometheus Panta is very versatile and can be used in a number of contexts: ![]() With the new Prometheus Panta device it is possible for the first time to measure colloidal (DLS) and conformational (nanoDSF/DLS) stability in one experiment with minimal sample volume (10 µl) and time (1.5 hours for 48 capillaries with nanoDSF and DLS data). This enables the rapid measurement of particles of different types and chemistry in solution and without expensive sample pre-treatment: Proteins, peptides, nucleic acids, viruses, micelles, lipids, liposomes, aggregations, emulsions can be measured.Ĭonsequently, DLS is a great tool for buffer and formulation screening as well as screening of small molecule compound libraries for influence on colloidal protein stability and aggregation tendency. Dynamic light scattering utilizes the elastic scattering of light (also known as Raleigh scattering) to determine the diffusion coefficients and hence the hydrodynamic radii of macromolecules. DLS stands for dynamic light scattering and is the method of choice for particle sizing and optimization of colloidal sample stability.
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